It is difficult to know exactly when the shift from gelatine to agar occurred. As often happens for scientific breakthroughs, agar was likely adopted incrementally alongside the use of other growth media. In 1913, for example, the first diagnosis of Serratia marcescens as a human pathogen was made by growing it on agar as well as on potatoes.
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Under certain circumstances, it’s possible to modify ordered dithering so that it can better handle colour information. This requires that we use a palette that is regularly distributed in colour space. A regular palette is composed of all possible combinations of red, green and blue tristimulus values, where each colour component is partitioned into a number of equally spaced levels. For example, 6 levels of red, green and blue totals 6³ = 216 unique colours, equivalent to the common web-safe palette.