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The spellbound astronauts pinned themselves to the windows, the first humans to see the far side of our nearest celestial neighbour. And then, from over the advancing horizon, an incredible sight.
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Funding limitations prevented Nyren from dedicating much time to the project at first, but in 1993, he was finally able to publish a proof-of-principle. His technique began by mixing the template DNA with a primer, a single dNTP, and three enzymes: the familiar DNA polymerase plus the light cascade enzymes, ATP sulfurylase and firefly luciferase.6 If the dNTP was incorporated into a strand of DNA, PPi would be produced in the chemical reaction. ATP sulfurylase could then convert the PPi into ATP, which would provide energy for the luciferase enzyme, producing light. Thus, it was possible to determine each base in the sequence by cycling through the dNTPs one at a time until light was detected, and then washing extra nucleotides out between each step. By literally rinsing and repeating, the sequence could be recorded one letter at a time without the use of any gels, which often took hours to run and were difficult to automate.，详情可参考纸飞机下载
过去，很多主打视频的旗舰手机，往往只有那颗底最大的主摄能拍出高质量画面。一旦切换到超广角或者长焦，画质、色彩、动态范围就会出现肉眼可见的断崖式下跌，焦段之间的割裂感，简直是后期调色的噩梦。